AIF (N-TERM); RB X-100UG

Application

Western blot: 0.25 to 1 µg/mL.

K562 cell lysate from HeLa cells can be used as a positive control and a 67 kDa band should be detected.

Optimal working dilutions must be determined by end user.

Research CategoryApoptosis & Cancer

Research Sub CategoryApoptosis - Additional

This Anti-AIF Antibody, N-terminus is validated for use in WB for the detection of AIF.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Rabbit anti-AIF (N-terminus of mature AIF) polyclonal antibody was raised against a peptide corresponding to amino acids 109 to 122 of human AIF (Susin et al. 1999).

Epitope: N-terminus

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 04-430

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified IgG by ion exchange chromatography. Liquid in PBS containing 0.02% sodium azide.

Format: Purified

Specificity

Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of the caspase family, caspase activated DNase, and several novel proteins (Zamzami & Kroemer 1999). A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF) (Susin et al. 1999). Like the critical molecules, cytochrome c and caspase 9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins, cytochrome c and caspase-9. AIF induces chromatin condensation and DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection. AIF is highly conserved between human and mouse and is widely expressed (Susin et al. 1999).

Storage and Stability

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.